 | REAL SC | 18/09/26 - 21:30 1 - 4 | ACADEMICA (Portugal - Segunda Liga) |  |
| ACDR LAMELAS | 19/09/26 - 18:00 4 - 1 | SANTO ANTONIO | ||
 | ATLETICO CP | 19/09/26 - 16:00 4 - 1 | LAGOA | |
| BEIRA MAR | 19/09/26 - 21:00 0 - 2 | BELENENSES |  |
| CASTRO DAIRE | 19/09/26 - 15:00 0 - 1 | PORTIMONENSE (Portugal - Segunda Liga) |  | |
| IMORTAL | 19/09/26 - 16:00 1 - 5 | TONDELA (Portugal - Segunda Liga) |  | |
 | MAFRA | 19/09/26 - 19:00 2 - 2 (4-3 PEN) | TIRSENSE | |
| NAVAL 1893 | 19/09/26 - 16:00 0 - 2 | FELGUEIRAS 1932 (Portugal - Segunda Liga) |  | |
| O ELVAS | 19/09/26 - 19:00 0 - 2 | UNIAO LEIRIA (Portugal - Segunda Liga) |  |
| SALGUEIROS | 19/09/26 - 16:00 1 - 0 | FEIRENSE (Portugal - Segunda Liga) |  | |
 | UD OLIVEIRENSE | 19/09/26 - 18:00 1 - 2 | PENAFIEL (Portugal - Segunda Liga) |  |
| VITORIA DE SERNACHE | 19/09/26 - 16:00 2 - 1 | VARZIM |  |
| AD MARCO 09 | 20/09/26 - 18:00 1 - 0 | MAIA LIDADOR | ||
| ALCAINS | 20/09/26 - 16:00 0 - 2 | SETUBAL | ||
| ALCOCHETENSE | 20/09/26 - 16:00 2 - 0 | CF BENFICA | ||
| ALPENDORADA | 20/09/26 - 16:00 3 - 0 | MACAO | ||
| AMORA | 20/09/26 - 16:00 3 - 4 | UNIAO DA SERRA | |
| ANCA | 20/09/26 - 16:00 1 - 4 | SANJOANENSE |  | |
| BOMBARRALENSE | 20/09/26 - 16:00 0 - 1 | VIZELA (Portugal - Segunda Liga) |  | |
| BRAGANCA | 20/09/26 - 16:00 6 - 1 | CELORICENSE | ||
| CINFAES | 20/09/26 - 15:00 0 - 4 | LEIXOES (Portugal - Segunda Liga) |  | |
| ESTRELA CALHETA | 20/09/26 - 13:00 1 - 3 | VIANENSE | ||
 | FAFE | 20/09/26 - 11:00 4 - 0 | LAJENSE | |
| FATIMA | 20/09/26 - 16:00 4 - 1 | VINHAIS | |
| FAZENDENSE | 20/09/26 - 16:00 6 - 1 | FIGUEIRENSE |  | |
| FC MADALENA | 20/09/26 - 16:00 1 - 3 | LUSITANIA LOUROSA (Portugal - Segunda Liga) |  | |
| GUARDA FC | 20/09/26 - 16:00 2 - 3 | CHAVES (Portugal - Segunda Liga) |  | |
| MORTAGUA | 20/09/26 - 16:00 0 - 2 | LECA | | |
 | OLHANENSE | 20/09/26 - 16:00 0 - 0 (1-0 ET) | PORTEL | |
| OLIVEIRA HOSPITAL | 20/09/26 - 16:00 1 - 1 (3-2 EY) | JUVENTUDE EVORA | ||
| OS LIMIANOS | 20/09/26 - 16:00 3 - 0 | ALJUSTRELENSE | |
| OVARENSE | 20/09/26 - 16:00 0 - 1 | OS MARIALVAS | ||
| PAREDES | 20/09/26 - 17:00 5 - 1 | MARIA DA FONTE | | |
| PONTE DA BARCA | 20/09/26 - 16:00 0 - 2 | AMARANTE (Portugal - Segunda Liga) |  | |
| SACAVENENSE | 20/09/26 - 12:00 0 - 1 | FARENSE (Portugal - Segunda Liga) |  |
| SANTAREM | 20/09/26 - 16:00 5 - 0 | MERELINENSE | | |
| SERPA | 20/09/26 - 16:00 1 - 1 (1.2 ET) | LOULETANO | | |
| SINTRENSE | 20/09/26 - 16:00 0 - 2 | AFS (Portugal - Segunda Liga) |  | |
| VILA POUCA | 20/09/26 - 16:00 0 - 5 | BRITO | ||
| CAMACHA | 27/09/26 - 16:00 0 - 1 | FLORGRADE |
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