| ACV | 22/09/26 - 20:00 2 - 1 | HOOGEVEEN | ||
| EEMDIJK | 22/09/26 - 20:00 2 - 1 | ROOSENDAAL |  | |
| EVV | 22/09/26 - 20:00 3 - 1 | HALSTEREN | ||
| EXCELSIOR 31 | 22/09/26 - 20:00 4 - 3 | AFC 34 | |
| GROENE STER | 22/09/26 - 20:00 1 - 6 | AFC | |
 | GVVV | 22/09/26 - 20:00 4 - 0 | DVS 33 | |
| HARDENBERG | 22/09/26 - 20:00 0 - 1 | BARENDRECHT | ||
| HFC | 22/09/26 - 20:00 1 - 1 (3-1 ET) | VV DOVO | ||
| KLOETINGE | 22/09/26 - 20:00 8 - 0 | ACHILLES VEEN | | |
| NOORDWIJK | 22/09/26 - 20:00 1 - 0 | FRISIA | |
| RIJNVOGELS | 22/09/26 - 20:00 0 - 1 | VVSB |  | |
| RKAV VOLENDAM | 22/09/26 - 20:00 2 - 2 (4-3 PEN) | SPAKENBURG | | |
| SPORTLUST | 22/09/26 - 20:00 0 - 2 | EXCELSIOR MAASSLUIS | | |
| STAPHORST | 22/09/26 - 20:00 2 - 3 | GENEMUIDEN | ||
| GROESBEEK | 23/09/26 - 20:00 1 - 5 | KOZAKKEN BOYS |  | |
 | IJSSELMEERVOGELS | 23/09/26 - 20:00 0 - 3 | GEMERT | |
| SPARTA NIJKERK | 23/09/26 - 20:00 3 - 1 | UDI 19 | |
| TOGB | 23/09/26 - 20:00 4 - 6 | RAALTE | ||
| VV ZWALUWEN | 23/09/26 - 20:00 0 - 1 | PURMERSTEIJN | ||
| WATERGRAAFSMEER | 23/09/26 - 20:00 2 - 2 (4-3 PEN) | TEC |
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